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Campylobacter jejuni Serotype HS:10 Capsular Polysaccharide and the Conjugate Vaccine thereof

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dc.contributor.advisor Monteiro, Mario A DePass, Christina Marie 2011-12-09 2011-12-14T20:13:59Z 2012-12-10T13:40:25Z 2011-12-14
dc.description.abstract Campylobacter jejuni (C. jejuni) is a food-borne bacterial pathogen that is the leading cause of Traveller’s Diaharea and Guillian-Barré Syndrome. Previous research determined that bacterial surface carbohydrates are virulence factors. Specifically, the study of the capsular polysaccharide (CPS) structures has widespread applications to understanding serotype cross-reactivity and biosynthetic pathways, the function of bacterial surface carbohydrates, and glycoconjugate vaccine development. This thesis characterized the CPS of C. jejuni HS:10 and subsequently used the CPS for developing a glycoconjugate vaccine. This was accomplished through extraction and purification of sugar from the bacteria, followed by characterization using chemical degradation, GC-MS, NMR and conjugation techniques. These analyses determined that the CPS was composed of a 3-linked GalNAc backbone and 6d-Galhepf attached at the 4th position with non-stoichiometrically attached O-methyl phosphoramidate attached to the 3 position of the heptose. Using this structure, a successful glycoconjugate vaccine was prepared using periodate oxidation and reductive amination. en_US
dc.language.iso en en_US
dc.rights.uri *
dc.subject Campylobacter en_US
dc.subject capsular polysaccharide en_US
dc.subject vaccine en_US
dc.subject pathogen en_US
dc.title Campylobacter jejuni Serotype HS:10 Capsular Polysaccharide and the Conjugate Vaccine thereof en_US
dc.type Thesis en_US Chemistry en_US Master of Science en_US Department of Chemistry en_US

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