Long-Term Monensin Supplementation Does Not Significantly Affect the Quantity or Diversity of Methanogens in the Rumen of the Lactating Dairy Cow

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Long-Term Monensin Supplementation Does Not Significantly Affect the Quantity or Diversity of Methanogens in the Rumen of the Lactating Dairy Cow

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dc.contributor.author Hook, Sarah E.
dc.contributor.author Northwood, Korinne S.
dc.contributor.author Wright, André-Denis G.
dc.contributor.author McBride, Brian W.
dc.date.accessioned 2009-08-04T14:24:41Z
dc.date.available 2009-08-04T14:24:41Z
dc.date.issued 2009-01
dc.identifier.citation APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 2009, p. 374–380 Vol. 75, No. 2 en
dc.identifier.other doi:10.1128/AEM.01672-08
dc.identifier.uri http://hdl.handle.net/10214/1992
dc.description.abstract A long-term monensin supplementation trial involving lactating dairy cattle was conducted to determine the effect of monensin on the quantity and diversity of rumen methanogens in vivo. Fourteen cows were paired on the basis of days in milk and parity and allocated to one of two treatment groups, receiving (i) a control total mixed ration (TMR) or (ii) a TMR with 24 mg of monensin premix/kg of diet dry matter. Rumen fluid was obtained using an ororuminal probe on day 15 (baseline) and days 20, 90, and 180 following treatment. Throughout the 6-month experiment, the quantity of rumen methanogens was not significantly affected by monensin supplementation, as measured by quantitative real-time PCR. The diversity of the rumen methanogen population was investigated using denaturing gradient gel electrophoresis (DGGE) and 16S rRNA clone gene libraries. DGGE analysis at each sampling point indicated that the molecular diversity of rumen methanogens from monensin-treated cattle was not significantly different from that of rumen methanogens from control cattle. 16S rRNA gene libraries were constructed from samples obtained from the rumen fluids of five cows, with a total of 166 clones examined. Eleven unique 16S rRNA sequences or phylotypes were identified, five of which have not been recognized previously. The majority of clones (98.2%) belonged to the genus Methanobrevibacter, with all libraries containing Methanobrevibacter strains M6 and SM9 and a novel phylotype, UG3322.2. Overall, long-term monensin supplementation was not found to significantly alter the quantity or diversity of methanogens in the rumens of lactating dairy cattle in the present study. en
dc.description.sponsorship Support from the Natural Science and Engineering Research Council (to B.W.M.) and the Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA) is gratefully acknowledged. en
dc.language.iso en en
dc.publisher Applied and Environmental Microbiology en
dc.subject methane en
dc.subject methanogen en
dc.subject monensin en
dc.subject rumen en
dc.title Long-Term Monensin Supplementation Does Not Significantly Affect the Quantity or Diversity of Methanogens in the Rumen of the Lactating Dairy Cow en
dc.type Article en


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