Differential regulation of components of the apoptotic and ubiquitin-mediated proteolytic pathway in slow- and fast-twitch muscle type in lambs receiving increasing amounts of dietary non-structural carbohydrate

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Differential regulation of components of the apoptotic and ubiquitin-mediated proteolytic pathway in slow- and fast-twitch muscle type in lambs receiving increasing amounts of dietary non-structural carbohydrate

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dc.contributor.author Greenwood, S.L.
dc.contributor.author Steele, M.
dc.contributor.author AlZahal, O.
dc.contributor.author Hook, S.E.
dc.contributor.author Swanson, K.C.
dc.contributor.author McBride, B.W.
dc.date.accessioned 2010-10-04T15:22:02Z
dc.date.available 2010-10-04T15:22:02Z
dc.date.issued 2010-09
dc.identifier.citation Greenwood et al. (2010) CJAS 90(3):421-428 en
dc.identifier.issn 1918-1825
dc.identifier.uri http://hdl.handle.net/10214/2264
dc.description.abstract Non-fibre carbohydrate (NFC) rich diets are commonly fed in ruminant production systems. The objective was to determine whether NFC challenge affects proteasome activity, or messenger ribonucleic acid (mRNA) expression of proteasome subunits or apoptotic Bcl-2 proteins, in slow- or fast- twitch muscle of sheep. For 12 d prior to slaughter, lambs (n = 8) received either a control diet (28.4% of dry matter as grain), or a diet of increasing amounts of grain up to 79.1% of dry matter. A decrease in urinary pH (P = 0.01), base excess of blood and extracellular fluid (P = 0.01), bicarbonate (P = 0.03) and total carbon dioxide (P = 0.04), and an increase in anion gap (P = 0.07) in NFC lambs are indicative of metabolic acidosis. NFC lambs had significantly lower mRNA expression of the 20S -β subunit (P = 0.05), and a tendency toward lower mRNA expression of the 20S-α subunit (P = 0.11) and the 19S isoform (P = 0.15) in soleus, but not to the same extent in extensor digitorum longus (EDL) muscle. Downregulation of Bad mRNA expression occurred in both soleus (P = 0.10) and EDL (P = 0.08) muscle as a result of NFC challenge. These results indicate that NFC challenge does affect mRNA expression of genes related to the proteasome and apoptosis in a muscle specific manner. en
dc.language.iso en en
dc.publisher Canadian Journal of Animal Science en
dc.relation.ispartofseries 90;3
dc.subject Ubiquitin-mediated proteolytic pathway, non-fibre carbohydrate, apoptosis, sheep en
dc.title Differential regulation of components of the apoptotic and ubiquitin-mediated proteolytic pathway in slow- and fast-twitch muscle type in lambs receiving increasing amounts of dietary non-structural carbohydrate en
dc.type Article en


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