Whole genome amplification of the rust Puccinia striiformis f. sp. tritici from single spores

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Whole genome amplification of the rust Puccinia striiformis f. sp. tritici from single spores

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dc.contributor.author Wang, Yanchun
dc.contributor.author Zhu, Mingqi
dc.contributor.author Zhang, Rong
dc.contributor.author Yang, Hanli
dc.contributor.author Wang, Yang
dc.contributor.author Guangyu, Sun
dc.contributor.author Jin, Shelin
dc.contributor.author Hsiang, Tom
dc.date.accessioned 2010-12-27T19:18:45Z
dc.date.available 2010-12-27T19:18:45Z
dc.date.issued 2009
dc.identifier.citation Wang, Y., Zhu, M., Zhang, R., Yang, H., Wang, Y., Guangyu, S., Jin, S., and Hsiang, T. "Whole genome amplification of the rust Puccinia striiformis f. sp. tritici from single spores." Journal of Microbiological Methods 77.2 (2009): 229-334
dc.identifier.uri http://hdl.handle.net/10214/2388
dc.description.abstract Rust fungi are obligate parasites and cannot be routinely cultured to obtain sufficient biomass for DNA extractions. Multiple displacement amplification (MDA) was demonstrated in this study for whole genome amplification from single spores of the rust fungus, Puccinia striiformis. The genomic DNA coverage and fidelity of this method was evaluated by PCR amplification and sequencing of two genetic markers: portions of the multi-copy nuclear ribosomal DNA internal transcribed spacer region (ITS) and the single copy β-tubulin gene from two geographical diverse isolates. Our results show that MDA is a valuable tool for whole genome amplification from single spores, and we propose that MDA-amplified DNA can be used for molecular genetic analysis of the wheat yellow rust fungus.
dc.language.iso en en_US
dc.publisher Journal of Microbiological Methods en_US
dc.subject genome amplification en_US
dc.subject Puccinia striiformis en_US
dc.subject spores en_US
dc.subject Rust en_US
dc.subject Fungi en_US
dc.title Whole genome amplification of the rust Puccinia striiformis f. sp. tritici from single spores en_US
dc.type Article en_US


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