Development of a method to generate a soluble substrate for lytic transglycosylases

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Development of a method to generate a soluble substrate for lytic transglycosylases

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dc.contributor.advisor Clarke, Anthony J.
dc.contributor.author Mark, Adam L.
dc.date 2011-04-11
dc.date.accessioned 2011-04-18T12:44:09Z
dc.date.available 2011-04-18T12:44:09Z
dc.date.issued 2011-04-18
dc.identifier.uri http://hdl.handle.net/10214/2644
dc.description This thesis was typeset with LaTeX using Minion Pro and Myriad Pro typefaces. en_US
dc.description.abstract Peptidoglycan, the major component of the bacterial cell wall, is essential for cell viability. Several important antibiotics disrupt peptidoglycan metabolism, including the β-lactams and vancomycin. There are several bacterial enzymes involved in peptidoglycan metabolism that are not yet the target of antibiotics, such as the lytic transglycosylases (LTs). Relatively little experimental characterization has been done on LTs, due largely to the difficulties of working with insoluble, heterogeneous, and highly variable peptidoglycan. This research develops a method for the generation of a soluble, homogeneous oligosaccharide substrate that can be used to study LTs. The approach taken was based on the enzymatic degradation of peptidoglycan into fragments of a specific nature, and their separation by HPLC. This work identifies the challenges associated with this approach, and discusses the potential flaws in the 'top-down' generation of a soluble substrate. en_US
dc.language.iso en en_US
dc.rights.uri http://creativecommons.org/licenses/by/3.0/ *
dc.subject Peptidoglycan en_US
dc.subject Lytic transglycosylase en_US
dc.subject HPAEC-PAD en_US
dc.subject MALDI-TOF MS en_US
dc.subject Oligosaccharide en_US
dc.subject Bacterial cell wall en_US
dc.title Development of a method to generate a soluble substrate for lytic transglycosylases en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


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