PLANT RESPONSES TO ABIOTIC STRESSES: TWO MOLECULAR APPROACHES IN ARABIDOPSIS AND MAIZE

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PLANT RESPONSES TO ABIOTIC STRESSES: TWO MOLECULAR APPROACHES IN ARABIDOPSIS AND MAIZE

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dc.contributor.advisor Rothstein, Steven J.
dc.contributor.author Humbert, Sabrina
dc.date 2011-08-11
dc.date.accessioned 2011-08-25T20:06:45Z
dc.date.available 2011-08-25T20:06:45Z
dc.date.issued 2011-08-25
dc.identifier.uri http://hdl.handle.net/10214/2867
dc.description.abstract Abiotic stress is highly detrimental to crop productivity worldwide. Research is key to meeting the challenges of modern agriculture in a sustainable and positive fashion. This thesis contributes to our understanding of plant stress responses by examining two molecular aspects of abiotic stress. The first part of the work focused on the Unfolded Protein Response (UPR), a general stress response mechanism triggered by the accumulation of unfolded or misfolded proteins in the endoplasmic reticulum. The study was conducted with the model plant Arabidopsis and shed new light on key players in the pathway. An unconventional alternative splicing mechanism, similar to the one identified in other higher eukaryotes, was found to parallel the activation of an ER-resident chaperone. The data suggest that this event is important to alleviate cellular stress in response to adverse environmental conditions such as heat. Further understanding of this pathway will help to develop a more complete view of the mechanisms involved in this response. The second part of the work investigated the interaction between nitrogen limitation and drought at the transcriptional level. A genome-wide transcript profiling experiment was performed to provide a comprehensive view of the response to nitrogen and water limitation in corn. The main finding was the demonstration of a clear synergistic effect between both stresses, an effect that was unexpectedly as important as either stress applied alone. This study adds to our current knowledge of abiotic stress response in plants and should provide the groundwork necessary to build future strategies for crop enhancement. en_US
dc.language.iso en en_US
dc.subject plant en_US
dc.subject nitrogen en_US
dc.subject drought en_US
dc.subject unfolded protein response en_US
dc.subject heat en_US
dc.subject abiotic stress en_US
dc.subject Arabidopsis en_US
dc.subject maize en_US
dc.subject microarray en_US
dc.title PLANT RESPONSES TO ABIOTIC STRESSES: TWO MOLECULAR APPROACHES IN ARABIDOPSIS AND MAIZE en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Doctor of Philosophy en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


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