Development of an inducible and reversible mouse model of podocyte effacement

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Development of an inducible and reversible mouse model of podocyte effacement

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dc.contributor.advisor Jones, Nina
dc.contributor.author Stringer, Colin D.M.
dc.date 2011-08-26
dc.date.accessioned 2011-08-31T20:25:58Z
dc.date.available 2011-08-31T20:25:58Z
dc.date.issued 2011-08-31
dc.identifier.uri http://hdl.handle.net/10214/2935
dc.description.abstract Podocytes are specialized epithelial cells which wrap glomerular capillaries with numerous interdigitating foot processes (FP). Between adjacent FPs a unique junction, the slit diaphragm (SD), functions as the final blood filtration barrier. Actin organization is critical for maintaining FP structure and SD function, and the adaptor protein Nck can bind an intracellular SD component to couple it with actin regulators. Podocyte-specific deletion of Nck in mice results in proteinuria and FP effacement. To better understand FP remodelling, we have pursued a transgenic mouse model utilizing an inducible and reversible dominant negative Nck (DN-Nck) to prevent signalling to actin regulators, exclusively in podocytes. Effects of DN-Nck were first confirmed in vitro, and transgenic mice were then generated and induced to express DN-Nck. Despite obtaining several mice which exhibited a mild renal phenotype, transgene expression appeared to be lost in successive generations. Full in vivo analysis awaits generation of additional transgenic founders. en_US
dc.language.iso en en_US
dc.subject nephropathy, proteinuria, glomerular filtration, podocyte, slit diaphragm, Nck, transgenic mouse model en_US
dc.title Development of an inducible and reversible mouse model of podocyte effacement en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


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