Structural responses of Daucus carota root-organ cultures and the arbuscular mycorrhizal fungus, Glomus intraradices, to 12 pharmaceuticals

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Structural responses of Daucus carota root-organ cultures and the arbuscular mycorrhizal fungus, Glomus intraradices, to 12 pharmaceuticals

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dc.contributor.author Hillis, Derek G.
dc.contributor.author Antunes, Pedro
dc.contributor.author Sibley, Paul K.
dc.contributor.author Klironomos, John N.
dc.contributor.author Solomon, Keith R.
dc.date.accessioned 2011-09-14T18:44:08Z
dc.date.available 2011-09-14T18:44:08Z
dc.date.issued 2008
dc.identifier.citation Hillis, D.G., Antunes, P., Sibley, P.K., Klironomos, J.N., Solomon, K.R. "Structural responses of Daucus carota root-organ cultures and the arbuscular mycorrhizal fungus, Glomus intraradices, to 12 pharmaceuticals." Chemosphere 73.3 (2008): 344-352
dc.identifier.other http://dx.doi.org/10.1016/j.chemosphere.2008.05.063
dc.identifier.uri http://hdl.handle.net/10214/2993
dc.description.abstract Pharmaceuticals and personal care products may enter the terrestrial environment through the amendment of agricultural soils with manure or biosolids with potential impacts on beneficial soil microbe populations. The beneficial symbiotic relationship between most plant species and arbuscular mycorrhizal fungi is a primary determinant of plant health and soil fertility. As such, there is increasing recognition of the need to study the impacts of anthropogenic stressors on plant–microbe interactions in soil ecotoxicology studies and risk assessment. A case study exploring the use of root-organ cultures to evaluate the effects of 12 common veterinary and human-use pharmaceuticals on the arbuscular mycorrhizal fungus, Glomus intraradices grown on Daucus carota root-organ cultures is presented. The bioassays were conducted over a 28-day exposure period at concentrations up to 1000 lg l 1. Root length and the fungal endpoints of hyphal growth and spore production were evaluated weekly during the study. Sulfamethoxazole and atorvastatin were the most phytotoxic compounds with EC50 values of 45 lg l 1 and 65 lg l 1, respectively. Three compounds exhibited selective mycotoxicity, whereby the fungal symbiont was adversely affected at concentrations significantly less than that calculated for root length. The EC50 for G. intraradices hyphal length was 45 lg l 1 for doxycycline, while carbamazepine and 17-a-ethynyl estradiol targeted spore production with EC50 values of 113 and 116 lg l 1, respectively. The assay results indicate that the root lengths responded quickly to the presence of phytotoxic pharmaceuticals in the culture medium. Hyphal length is a sensitive endpoint after 21 days exposure, while spore production requires 28 days exposure before significant differences could be detected. Root-organ cultures provide an effective means to evaluate chemical stressors on arbuscular mycorrhizal fungi and can be used to screen for root-based phytotoxicity.
dc.language.iso en en_US
dc.publisher Chemosphere en_US
dc.subject Root-organ cultures en_US
dc.subject Arbuscular mycorrhizal fungi en_US
dc.subject pharmaceuticals en_US
dc.subject personal care products en_US
dc.subject phytotoxicity en_US
dc.subject Mycotoxicity en_US
dc.title Structural responses of Daucus carota root-organ cultures and the arbuscular mycorrhizal fungus, Glomus intraradices, to 12 pharmaceuticals en_US
dc.type Article en_US


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