Biodegradation of Polycyclic Aromatic Hydrocarbons by Arthrobacter sp. UG50 Isolated from Petroleum Refinery Wastes

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Biodegradation of Polycyclic Aromatic Hydrocarbons by Arthrobacter sp. UG50 Isolated from Petroleum Refinery Wastes

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dc.contributor.advisor Trevors, Jack T.
dc.contributor.advisor Lee, Hung
dc.contributor.advisor Stehmeier, Les
dc.contributor.author Koch, Elisabeth
dc.date 2009-10-20
dc.date.accessioned 2011-11-21T19:48:24Z
dc.date.available 2011-11-21T19:48:24Z
dc.date.issued 2011-11-21
dc.identifier.uri http://hdl.handle.net/10214/3140
dc.description.abstract North American petroleum refineries use landfarming to dispose of hydrocarbon-containing wastes for bioremediation by indigenous soil microorganisms. In this study, we isolated PAH-degrading bacteria from landfarm soil by enrichment with hydrocarbon-containing effluent. One isolate, Arthrobacter sp. UG50, was capable of using phenanthrene and anthracene as sole carbon sources. The strain degraded phenanthrene (200 mg/L) within 24 h in pure culture at high cell density (10e8 cells/mL). Anthracene (50 mg/L) was slowly degraded, with 29% degraded within 21 days. The strain could not use naphthalene, pyrene, chrysene or benzo(a)pyrene as sole carbon sources, but could degrade pyrene (50 mg/L) cometabolically when phenanthrene was provided. Anthracene degradation (50 mg/L) was enhanced by phenanthrene, with 100% degraded within 6 days. The addition of strain UG50 to petroleum sludge in baffled flasks increased total hydrocarbon degradation and degradation of low concentrations of fluorene, phenanthrene, pyrene and chrysene compared to flasks with limited aeration or containing sludge alone. en_US
dc.description.sponsorship NOVA Chemicals and the Ontario Centres of Excellence en_US
dc.language.iso en en_US
dc.subject Polycyclic Aromatic Hydrocarbons en_US
dc.subject Biodegradation en_US
dc.subject Arthrobacter en_US
dc.subject Slurry Bioreactors en_US
dc.subject Landfarming en_US
dc.subject Petroleum Refinery Wastes en_US
dc.title Biodegradation of Polycyclic Aromatic Hydrocarbons by Arthrobacter sp. UG50 Isolated from Petroleum Refinery Wastes en_US
dc.type Thesis en_US
dc.degree.programme Environmental Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Environmental Biology en_US


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