The formation of androstenone conjugates from testes tissue of the mature boar.

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The formation of androstenone conjugates from testes tissue of the mature boar.

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dc.contributor.advisor Squires, E.J.
dc.contributor.author Desnoyer, Jillian Eve
dc.date 2011-11-22
dc.date.accessioned 2011-12-01T20:02:09Z
dc.date.available 2011-12-01T20:02:09Z
dc.date.issued 2011-12-01
dc.identifier.uri http://hdl.handle.net/10214/3165
dc.description.abstract The accumulation of androstenone in the fat of mature boars results in boar taint; the conjugation of androstenone would decrease this important meat quality problem by decreasing the accumulation and increasing the excretion of androstenone. Leydig cells and testis microsomes from mature boars were incubated with radiolabeled pregnenolone, and the free and conjugated metabolites were examined by HPLC. Sulfated androstenone with a mass of 367 m/z was directly identified by MS, with a novel tentative structure of 3-keto-4- sulfoxy-androstenone. Addition of enolase to the microsomal incubations increased the formation of 3-keto-4-sulfoxy-androstenone. Overexpression of SULT2A1 in HEK cells resulted in the sulfoconjugation of dehydroepiandrosterone, but not androstenone, suggesting that SULT2A1 may not be involved in sulfoconjugation of androstenone. This thesis describes the novel direct characterization of androstenone sulfate and the importance of enolase in its formation. The relevance to boar taint metabolism is discussed. en_US
dc.language.iso en en_US
dc.subject androstenone en_US
dc.subject conjugate en_US
dc.subject SULT2A1 en_US
dc.subject boar taint en_US
dc.subject androstene en_US
dc.subject cloning en_US
dc.subject enolase en_US
dc.subject HPLC en_US
dc.subject Leydig cell en_US
dc.subject sulfoconjugation en_US
dc.subject sulfation en_US
dc.subject adipocytes en_US
dc.subject porcine en_US
dc.subject pig en_US
dc.subject metabolism en_US
dc.title The formation of androstenone conjugates from testes tissue of the mature boar. en_US
dc.type Thesis en_US
dc.degree.programme Animal and Poultry Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Animal and Poultry Science en_US


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