Regulation of prostaglandin synthesis in the zebrafish ovary

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Regulation of prostaglandin synthesis in the zebrafish ovary

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dc.contributor.advisor Van Der Kraak, Glen
dc.contributor.author Melnyk, Nicholas C.
dc.date 2011-12-15
dc.date.accessioned 2011-12-21T19:04:20Z
dc.date.available 2011-12-21T19:04:20Z
dc.date.issued 2011-12-21
dc.identifier.uri http://hdl.handle.net/10214/3209
dc.description.abstract Oocyte maturation and ovulation are two major events that occur in fish prior to spawning. While earlier studies have shown that 17α, 20β-dihydroxy-4-pregnen-3-one (17,20β-P) and the insulin-like growth factor (IGF) system are regulators of oocyte maturation in the zebrafish (Danio rerio), it is not known whether these hormones play a role in regulating prostaglandin synthesis which is thought to mediate ovulation. I determined if 17,20β-P and human IGF-1 affect the expression of genes involved in prostaglandin biosynthesis including phospholipase A2 (cpla2) and cyclooxygenase-1/2 (ptgs1/ptgs2), or prostaglandin F2α (PGF2α) levels. 17,20β-P and IGF-1 stimulated oocyte maturation in mid-vitellogenic (MV) and full grown (FG) follicles. In FG follicles, 17,20β-P increased cpla2 expression, whereas IGF-1 increased cpla2 and ptgs2 expression. Both 17,20β-P and IGF-1 increased PGF2α production. The phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) signalling pathways were shown to mediate IGF-1- and 17,20β-P-induced oocyte maturation and cpla2 and ptgs2 expression. Collectively, these results demonstrate that 17,20β-P and IGFs are important regulators of oocyte maturation and prostaglandin synthesis in zebrafish. en_US
dc.language.iso en en_US
dc.subject Prostaglandin en_US
dc.subject Insulin-like growth factor en_US
dc.subject Maturation inducing steroid en_US
dc.subject Phospholipase en_US
dc.subject Cyclooxygenase en_US
dc.title Regulation of prostaglandin synthesis in the zebrafish ovary en_US
dc.type Thesis en_US
dc.degree.programme Integrative Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Integrative Biology en_US


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