Development of an in vitro Method to Determine Nickel Toxicity and Carcinogenicity

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Development of an in vitro Method to Determine Nickel Toxicity and Carcinogenicity

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dc.contributor.advisor Dutton, Roshni
dc.contributor.advisor Hayward, Gordon
dc.contributor.author Renaud, Matthew
dc.date 2012-04-30
dc.date.accessioned 2012-05-14T18:19:14Z
dc.date.available 2012-05-14T18:19:14Z
dc.date.issued 2012-05-14
dc.identifier.uri http://hdl.handle.net/10214/3632
dc.description.abstract Nickel is a widely used metal in industrial and commercial applications, being both workable and highly resistant to corrosion. Certain nickel compounds are known human carcinogens, but not all nickel compounds are equally hazardous. A robust method of assessing the carcinogenic potential of various nickel compounds is needed in order to determine safe occupational exposure levels. This work attempts to develop an in vitro mammalian cell culture method for assessing the carcinogenic and toxic potential of nickel compounds by metabolic and cell cycle analysis. Two cell lines, C3H/10T1/2 and MRC-5, were used. Measurements of extracellular metabolites by enzymatic methods and HPLC were combined with cell counts and cell cycle and apoptosis analysis by flow cytometry. This work shows that nickel sulphate can elicit a metabolic response similar to that of organic carcinogens, though the underlying mechanisms are likely different. en_US
dc.description.sponsorship Vale Canada Limited, NSERC en_US
dc.language.iso en en_US
dc.subject nickel en_US
dc.subject soluble nickel en_US
dc.subject insoluble nickel en_US
dc.subject nickel carcinogenicity en_US
dc.subject nickel toxicity en_US
dc.subject carcinogenicity en_US
dc.subject carcinogen testing en_US
dc.subject cell culture en_US
dc.subject nickel species en_US
dc.subject assays for carcinogenicity en_US
dc.subject short-term carcinogen testing en_US
dc.subject C3H/10T1/2 en_US
dc.subject MRC-5 en_US
dc.title Development of an in vitro Method to Determine Nickel Toxicity and Carcinogenicity en_US
dc.type Thesis en_US
dc.degree.programme Engineering en_US
dc.degree.name Master of Applied Science en_US
dc.degree.department School of Engineering en_US


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