An Anti-Clostridium difficile Vaccine: Chemical Synthesis of the Pentasaccharide Repeating Unit of Polysaccharide PS-I

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An Anti-Clostridium difficile Vaccine: Chemical Synthesis of the Pentasaccharide Repeating Unit of Polysaccharide PS-I

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dc.contributor.advisor Monteiro, Mario
dc.contributor.author Jiao, Yuening
dc.date 2012-06-18
dc.date.accessioned 2012-06-22T17:36:44Z
dc.date.available 2012-06-22T17:36:44Z
dc.date.issued 2012-06-22
dc.identifier.uri http://hdl.handle.net/10214/3748
dc.description.abstract Clostridium difficile is a Gram-positive bacterium that is the most common cause of hospital-associated and antimicrobial-associated diarrhea in humans. Monteiro and co-workers have discovered that C. difficile expresses three cell-surface polysaccharides, named PS-I, PS-II and PS-III. Interestingly, PS-I was determined to be present in a ribotype 027 strain, the ribotype responsible for recent deadly outbreaks worldwide. In this work, the total chemical synthesis of the PS-I pentasaccharide with a linker molecule by a linear synthesis strategy from four monosaccharide building blocks is described: α-L-Rhap-(1→3)-β-D-Glcp-(1→4)-α-D-Glcp-(1→2)-α-D-Glcp-(1→O(CH2)5NH2 3 ↑ 1 α-L-Rhap The synthesized PS-I pentasaccharide will be conjugated to a protein carrier for evaluation as an anti-C. difficile glycoconjugate vaccine. en_US
dc.language.iso en en_US
dc.subject Chemistry, Carbohydrate, Synthesis, Vaccine, Clostrium difficile en_US
dc.title An Anti-Clostridium difficile Vaccine: Chemical Synthesis of the Pentasaccharide Repeating Unit of Polysaccharide PS-I en_US
dc.type Thesis en_US
dc.degree.programme Chemistry en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Chemistry en_US


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