VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE

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VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE

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dc.contributor.advisor Guzman, Ernesto
dc.contributor.author McGowan, Janine
dc.date 2012-06-05
dc.date.accessioned 2012-07-18T14:28:58Z
dc.date.available 2012-07-18T14:28:58Z
dc.date.issued 2012-07-18
dc.identifier.uri http://hdl.handle.net/10214/3801
dc.description.abstract Originally described from the Asian honey bee, Apis cerana, the microsporidian Nosema ceranae is an obligate, intracellular parasite that has recently been discovered infecting the western honey bee, Apis mellifera. More research on the biology of N. ceranae as well as on the impact it may have on A. mellifera is greatly needed. However, conducting studies on N. ceranae is not only dependent on seasonal availability of Nosema spores, but also on reliable methods for determining spore viability. This study presents the results of using cryogenics to provide long term storage of viable N. ceranae spores and a differential staining procedure that details how to use bright field microscopy with the fluorescent viability dye, propidium iodide (PI), and the fluorescent stain, 4', 6-diamidino-2-phenylindole (DAPI) to differentiate viable and nonviable spores. Using these methods, it was found that freezing N. ceranae at -70 °C in 10% glycerol yielded the lowest mean rate of spore mortality after thawing (24.2% ± 2.2). en_US
dc.language.iso en en_US
dc.subject Apis mellifera, Nosema ceranae, viability, cryopreservation en_US
dc.title VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE en_US
dc.type Thesis en_US
dc.degree.programme Environmental Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Environmental Biology en_US


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