oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse

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oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse

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dc.contributor.advisor Ma, David
dc.contributor.author Monteiro, Jessica
dc.date 2012-07-24
dc.date.accessioned 2012-08-24T18:48:40Z
dc.date.available 2012-08-24T18:48:40Z
dc.date.issued 2012-08-24
dc.identifier.uri http://hdl.handle.net/10214/3870
dc.description.abstract The biological activity of α-linolenic acid (ALA) is poorly understood and primarily associated with its conversion to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). This study used the Δ6 desaturase knockout (D6KO) mouse, which lacks Δ6 desaturase and therefore cannot convert ALA, to evaluate the independent effects of ALA on preventing non-alcoholic fatty liver disease (NAFLD). First, the capacity of very long chain fatty acids to rescue the D6KO lipid profile was established. Next, to evaluate the independent effects of ALA, D6KO or wild-type mice were fed diets containing lard, canola, flaxseed, or fish oil. Following treatment, liver phospholipid fatty acid composition was evaluated and livers were scored for steatosis and inflammation. Glucose tolerance was also evaluated. D6KO mice fed ALA-rich diets had lower liver lipid accumulation, lower hepatic inflammation (8 weeks) and improved glucose tolerance (20 weeks) relative to lard-fed D6KO mice. Overall, this thesis supports an independent biological role for ALA. en_US
dc.description.sponsorship D.W.L. Ma is funded by the Canola Council of Canada, Natural Sciences and Engineering Research Council of Canada and the Canada Foundation for Innovation Leaders Opportunity Fund with matching from the Ontario Research Fund; Jessica Monteiro is funded Ontario Graduate Scholarship. en_US
dc.language.iso en en_US
dc.subject polyunsaturated fatty acid en_US
dc.subject alpha-linolenic acid en_US
dc.subject fatty liver disease en_US
dc.subject delta-6 desaturase en_US
dc.title oils rich in alpha-linolenic acid independently protect against characteristics of fatty liver disease in the delta-6 desaturase mouse en_US
dc.type Thesis en_US
dc.degree.programme Human Health and Nutritional Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Human Health and Nutritional Sciences en_US


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