Aspects of Nitrogen Metabolism in Symbiotic Cnidarians

The Atrium, University of Guelph Institutional Repository

Aspects of Nitrogen Metabolism in Symbiotic Cnidarians

Show simple item record

dc.contributor.advisor Ballantyne, James Stuart
dc.contributor.author Boutilier, Ryan Michael
dc.date 2012-05-30
dc.date.accessioned 2012-08-24T19:51:02Z
dc.date.available 2012-08-24T19:51:02Z
dc.date.issued 2012-08-24
dc.identifier.uri http://hdl.handle.net/10214/3874
dc.description.abstract The pathway of seawater ammonium assimilation and influence of light on amino acid synthesis remain unresolved in cnidarian symbioses. Labeled ammonium (10 μM 15NH4Cl) in seawater was used to trace the pathway of the incorporation into amino acids in host tissue, Zoanthus sp., and zooxanthellae, Symbiodinium microadriaticum. Freshly isolated zooxanthellae were exposed to 20 μM 15NH4Cl with coral homogenate to evaluate the role of host factors on amino acid synthesis. High performance liquid chromatography and mass spectrometry was used to measure percent labeling and concentrations of amino acids. In zooxanthellae, ammonium was assimilated into glutamine likely via glutamine synthetase and into glutamate via glutamine 2-oxoglutarate amidotransferase. Interrupting photosynthesis with DCMU did not inhibit glutamine and tryptophan synthesis however reduced the 15N-enrichment of glutamate, aspartate, and ornithine in zooxanthellae, as well as arginine, ornithine, and lysine in host tissue. Coral homogenate had little effect on the 15N-enrichment of glutamine, aspartate, and alanine in freshly isolated zooxanthellae. Evidence is presented to support the uptake of ammonium ions and data shows that glutamine and not glutamate is translocated to the coral host. en_US
dc.language.iso en en_US
dc.subject CORAL, ZOOXANTHELLAE, SYMBIOSIS, ZONATHUS, CNIDARIA, SYMBIODINIUM, GLUTAMINE, GLUTAMATE, TRYPTOPHAN, LCMS, TEM, DCMU, NITROGEN METABOLISM, AMMONIA, AMMONIUM en_US
dc.title Aspects of Nitrogen Metabolism in Symbiotic Cnidarians en_US
dc.type Thesis en_US
dc.degree.programme Integrative Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Integrative Biology en_US


Files in this item

Files Size Format View
Boutilier_Ryan_MSc_Thesis.pdf 4.853Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Search the Atrium


Advanced Search

Browse

My Account