Characterization and mapping of a wilting Zea mays mutant

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Characterization and mapping of a wilting Zea mays mutant

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dc.contributor.advisor Lukens, Lewis
dc.contributor.author Bruce, Robert
dc.date 2012-08-15
dc.date.accessioned 2012-09-04T14:20:47Z
dc.date.available 2013-08-15T05:00:19Z
dc.date.issued 2012-09-04
dc.identifier.uri http://hdl.handle.net/10214/3904
dc.description.abstract Understanding water stress responses in maize and the genetic factors controlling these traits has direct application to breeding and genetic study. To understand water stress responses in more detail, this thesis characterizes a novel B73 ethyl methanesulfonate induced wilting maize mutant. This mutant is shown to express a wilting phenotype under both field and greenhouse conditions, exhibiting leaf roll, stunted stem growth and reduced fertility. Germination is unaffected in mutant seeds and no abnormal seedling phenotypes are observed. Onset of the phenotype is between V3 and V6 growth stage, and corresponds with a disruption of stem elongation. To characterize the genetic elements underlying the wilting phenotype, F2 and F3 mapping populations were phenotyped and genotyped. The mutant phenotype was found at variable frequencies between populations; ¼ or less of all mapping population members were observed as wilting, implicating a single genetic factor controlling the mutant phenotype. Background effects of the non-mutant parent and residual EMS mutations are hypothesized to be responsible for the unexpected frequency of wilting observed. Single nucleotide polymorphism and microsatellite markers localized the wilted mutant allele to a 7.4-Megabase region on the long arm of chromosome 7. The phenotype results and rough mapping data provide a base for further study and cloning of the mutant allele. en_US
dc.language.iso en en_US
dc.subject maize en_US
dc.subject genetics en_US
dc.subject mutant en_US
dc.subject mapping en_US
dc.subject wilting en_US
dc.title Characterization and mapping of a wilting Zea mays mutant en_US
dc.type Thesis en_US
dc.degree.programme Plant Agriculture en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Plant Agriculture en_US


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