Understanding the Inheritance and Mechanism of Auxinic Herbicide Resistance in Wild Radish (Raphanus raphanistrum L.)

The Atrium, University of Guelph Institutional Repository

Understanding the Inheritance and Mechanism of Auxinic Herbicide Resistance in Wild Radish (Raphanus raphanistrum L.)

Show simple item record

dc.contributor.advisor Hall, J. Christopher
dc.contributor.author Di Meo, Natalie L.
dc.date 2012-09-11
dc.date.accessioned 2012-10-03T18:31:36Z
dc.date.available 2012-10-03T18:31:36Z
dc.date.issued 2012-10-03
dc.identifier.uri http://hdl.handle.net/10214/4039
dc.description.abstract Auxinic herbicide-resistant (i.e., resistant to 2,4-D and MCPA) wild radish (Raphanus raphanistrum L.) was discovered in the Western Australian wheatbelt, providing an opportunity to integrate auxinic herbicide resistance into cultivated radish (R. sativus L.) using conventional breeding methods. It was hypothesized that the inheritance of auxinic herbicide resistance in wild radish is conferred by a single, dominant nuclear gene and, therefore, will be relatively easy to introgress from wild radish to cultivated radish; and the mechanism of auxinic herbicide resistance in wild radish is through an altered target-site. Visual injury data of the F2 progeny suggested that resistance was conferred by a quantitative trait with the susceptible allele(s) exhibiting dominance with minor cytoplasmically inherited genes masking the susceptible trait. In conclusion, the resistance allele(s) were quantitative and, thus, make selection for resistance difficult. Therefore, the introgression of the resistance allele(s) was not successfully completed. To determine the mechanism of resistance, the wild radish plants resistant WARR6-26 (R) and susceptible WARR7-5 (S) were treated with radiolabeled MCPA. There was no difference in metabolism of [14C]MCPA between R and S plants. Based upon the decline in the total 14C recovered over 72 h in R and S it was clear that both were “losing” [14C]MCPA; however, R plants were losing MCPA more rapidly. It was hypothesized that because R plants exude 14C more rapidly from their roots than S plants, this accounted for the resistance of R plants. en_US
dc.language.iso en en_US
dc.subject auxinic herbicide en_US
dc.subject Raphanus en_US
dc.subject Raphanus raphanistrum en_US
dc.subject auxin en_US
dc.subject herbicide resistance en_US
dc.title Understanding the Inheritance and Mechanism of Auxinic Herbicide Resistance in Wild Radish (Raphanus raphanistrum L.) en_US
dc.type Thesis en_US
dc.degree.programme Environmental Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Environmental Biology en_US


Files in this item

Files Size Format View Description
Natalie.DiMeo - Thesis.pdf 2.125Mb PDF View/Open Thesis

This item appears in the following Collection(s)

Show simple item record

Search the Atrium


Advanced Search

Browse

My Account